Nature Communications (Jun 2020)

DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability

  • Kevin Kramm,
  • Tim Schröder,
  • Jerome Gouge,
  • Andrés Manuel Vera,
  • Kapil Gupta,
  • Florian B. Heiss,
  • Tim Liedl,
  • Christoph Engel,
  • Imre Berger,
  • Alessandro Vannini,
  • Philip Tinnefeld,
  • Dina Grohmann

DOI
https://doi.org/10.1038/s41467-020-16702-x
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 12

Abstract

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TATA-binding protein (TBP) and a transcription factor (TF) IIB-like factor are important constituents of all eukaryotic initiation complexes. Here, the authors use a DNA origami-based force clamp to investigate the assembly dynamics of human initiation complexes in the RNAP II and RNAP III systems at the single-molecule level under pico newton forces.