Modelling an experimental systemic pseudomonas infection process

Abstract

This article presents the results of an experimental model of a systemic pseudomonas aeruginosa infection. This model can be used to develop therapeutic measures to suppress an infectious disease caused by antibiotic-resistant strains of Pseudomonas aeruginosa. For this purpose, a dry lyophilised culture (strain name: Pseudomonas Aeruginosa No. 453, strain number 190158, obtained from the Vishnevsky Institute of Surgery. Pseudomonas aeruginosa culture was pre-cultured in test tubes with meat-peptone broth (MPB), after 30 min using a sterile pipette and 0.2 ml of sterile pipette was transferred to test tubes with meat-peptone agar (cetrimide agar) and cultured in anaerobic medium at 37±2 °C for 24 h at the thermostat. The Pseudomonas aeruginosa culture is resuspended by washing off the surface of the culture medium with 0.85% saline. In a sterile area, the culture is pipetted with a Pasteur pipette. The concentration of microbial cells is adjusted to the Tarasevich turbidity standard in a sterile test tube. Animals are held head down so that the viscera of the abdomen descend to the diaphragm. The injection is made in the lower third, to the left of the white line of the abdomen. The injection site is disinfected, a skin fold is taken and a needle is inserted into it, turned at right angles and the abdominal wall is punctured with a quick thrust. The needle is blunted beforehand to prevent damage to the intestinal loops. The volume of culture injected into rabbits and the clinical signs of infection depend on the concentration of microbial cells detected by the Tarasevich turbidity standard and the appropriate group. Conditions, selected in it, allow to create quickly the necessary concentration of microbial cells in abdominal cavity of laboratory animals, promoting peritonitis, and the criteria of performance are technically simple.