A new double signal on electrochemical aptasensor based on gold nanoparticles/graphene nanoribbons/MOF-808 as enhancing nanocomposite for ultrasensitive and selective detection of carbendazim
Hossein Khosropour,
Nattida Maeboonruan,
Chakrit Sriprachuabwong,
Adisorn Tuantranont,
Wanida Laiwattanapaisal
Affiliations
Hossein Khosropour
Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Biosensors and Bioanalytical Technology for Cells and Innovative Testing Device Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
Nattida Maeboonruan
Graphene and Printed Electronics for Dual-Use Applications Research Division (GPERD), National Security and Dual-Use Technology Center (NSD), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand
Chakrit Sriprachuabwong
Graphene and Printed Electronics for Dual-Use Applications Research Division (GPERD), National Security and Dual-Use Technology Center (NSD), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand
Adisorn Tuantranont
Graphene and Printed Electronics for Dual-Use Applications Research Division (GPERD), National Security and Dual-Use Technology Center (NSD), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand
Wanida Laiwattanapaisal
Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Biosensors and Bioanalytical Technology for Cells and Innovative Testing Device Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Corresponding author at: Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
The fungicide carbendazim (CBZ) is a carcinogen, which is widely found in the environment. It is a broad-spectrum benzimidazole fungicide that acts by disrupting fungal cell division. To detect CBZ in water samples, an ultrasensitive and selective dual-signal electrochemical aptasensing platform based on the interaction of SH-complementary carbendazim aptamer (SH-cCBZ) and carbendazim aptamer (CBZA) was developed. Gold nanoparticles (AuNP) modified with graphene nanoribbons (GNR), which were produced from multiwalled carbon nanotubes, and Zr-based metal-organic framework (MOF-808) (AuNP@GNR/MOF-808) were used for enhancing the signals for the first time. The SH-cCBZ produced a double-stranded structure along with CBZA. CBZ dispersed the double strands, thereby amplifying the electrochemical response. The electrochemical aptasensor showed a linear response to CBZ in the concentration range from 1.0 fmol L−1 to 100.0 pmol L−1 and 0.8 fmol L−1 to 100.0 pmol L−1 under ideal conditions. The detection limits obtained via electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) were 0.4 and 0.2 fmol L−1, respectively. Moreover, the proposed aptasensor was used to determine the CBZ levels in Bangkok tap water and Chao Phraya river water samples, the results were in good agreement with UPLC-MS/MS. The recoveries ranged from 97.5% to 102.8%, suggesting various potential applications.
