Intrinsically disordered protein biosensor tracks the physical-chemical effects of osmotic stress on cells

Cesar L. Cuevas-Velazquez; Tamara Vellosillo; Karina Guadalupe; Hermann Broder Schmidt; Feng Yu; David Moses; Jennifer A. N. Brophy; Dante Cosio-Acosta; Alakananda Das; Lingxin Wang; Alexander M. Jones; Alejandra A. Covarrubias; Shahar Sukenik; José R. Dinneny

doi:10.1038/s41467-021-25736-8

Nature Communications (Sep 2021)

Intrinsically disordered protein biosensor tracks the physical-chemical effects of osmotic stress on cells

Cesar L. Cuevas-Velazquez,
Tamara Vellosillo,
Karina Guadalupe,
Hermann Broder Schmidt,
Feng Yu,
David Moses,
Jennifer A. N. Brophy,
Dante Cosio-Acosta,
Alakananda Das,
Lingxin Wang,
Alexander M. Jones,
Alejandra A. Covarrubias,
Shahar Sukenik,
José R. Dinneny

Affiliations

Cesar L. Cuevas-Velazquez: Department of Biology, Stanford University
Tamara Vellosillo: Department of Biology, Stanford University
Karina Guadalupe: Center for Cellular and Biomolecular Machines (CCBM), University of California
Hermann Broder Schmidt: Department of Biochemistry, Stanford University School of Medicine
Feng Yu: Center for Cellular and Biomolecular Machines (CCBM), University of California
David Moses: Center for Cellular and Biomolecular Machines (CCBM), University of California
Jennifer A. N. Brophy: Department of Biology, Stanford University
Dante Cosio-Acosta: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México
Alakananda Das: Department of Molecular and Cellular Physiology, Stanford University
Lingxin Wang: Department of Molecular and Cellular Physiology, Stanford University
Alexander M. Jones: Sainsbury Laboratory, Cambridge University
Alejandra A. Covarrubias: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México
Shahar Sukenik: Center for Cellular and Biomolecular Machines (CCBM), University of California
José R. Dinneny: Department of Biology, Stanford University

DOI: https://doi.org/10.1038/s41467-021-25736-8
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Methods to monitor osmolarity-dependent changes in cell are currently lacking. Here the authors use the Arabidopsis intrinsically disordered AtLEA4-5 protein, which is expressed in plants under water deficit, to develop a FRET biosensor (SED1) to monitor osmotic stress.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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