Antiviral Activity of Benzoheterocyclic Compounds from Soil-Derived <i>Streptomyces jiujiangensis</i> NBERC-24992
Manli Liu,
Mengyao Ren,
Yani Zhang,
Zhongyi Wan,
Yueyin Wang,
Zhaoyuan Wu,
Kaimei Wang,
Wei Fang,
Xiliang Yang
Affiliations
Manli Liu
Hubei Biopesticide Engineering Research Centre, Hubei Academy of Agricultural Sciences, No. 8, Nanhu Ave., Hongshan District, Wuhan 430064, China
Mengyao Ren
Institute of Infection, Immunology and Tumor Microenvironment, Institute of Pharmaceutical Process, Department of Pharmacy, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Medicine, Wuhan University of Science and Technology, No. 2, Huangjiahu East Road, Hongshan District, Wuhan 430081, China
Yani Zhang
Hubei Biopesticide Engineering Research Centre, Hubei Academy of Agricultural Sciences, No. 8, Nanhu Ave., Hongshan District, Wuhan 430064, China
Zhongyi Wan
Hubei Biopesticide Engineering Research Centre, Hubei Academy of Agricultural Sciences, No. 8, Nanhu Ave., Hongshan District, Wuhan 430064, China
Yueyin Wang
Hubei Biopesticide Engineering Research Centre, Hubei Academy of Agricultural Sciences, No. 8, Nanhu Ave., Hongshan District, Wuhan 430064, China
Zhaoyuan Wu
Hubei Biopesticide Engineering Research Centre, Hubei Academy of Agricultural Sciences, No. 8, Nanhu Ave., Hongshan District, Wuhan 430064, China
Kaimei Wang
Hubei Biopesticide Engineering Research Centre, Hubei Academy of Agricultural Sciences, No. 8, Nanhu Ave., Hongshan District, Wuhan 430064, China
Wei Fang
Hubei Biopesticide Engineering Research Centre, Hubei Academy of Agricultural Sciences, No. 8, Nanhu Ave., Hongshan District, Wuhan 430064, China
Xiliang Yang
Institute of Infection, Immunology and Tumor Microenvironment, Institute of Pharmaceutical Process, Department of Pharmacy, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Medicine, Wuhan University of Science and Technology, No. 2, Huangjiahu East Road, Hongshan District, Wuhan 430081, China
Pseudorabies virus (PRV) is a pathogen that causes Aujeszky’s disease (AD) in animals, leading to huge economic losses to swine farms. In order to discover anti-PRV compounds, we studied the extracts of the strain Streptomyces jiujiangensis NBERC-24992, which showed significant anti-PRV activity. Eight benzoheterocyclic secondary metabolites, including three new compounds (1–3, virantmycins D–G) and five known compounds (4–8, virantmycin, A-503451 D, A-503451 D acetylate, A-503451 A, and A-503451 B), were isolated from the broth of NBERC-24992. The structures of the new compounds were identified by using extensive spectroscopic data, including mass spectrometry (MS), nuclear magnetic resonance (NMR), and electronic circular dichroism (ECD). Compound 1 was found to be a novel heterocyclic compound with a tricyclic skeleton from natural product. All compounds were tested for antiviral activity, and 4 (virantmycin) showed an excellent effect against PRV and was better than ribavirin and acyclovir. Our study revealed that chlorine atom and tetrahydroquinoline skeleton were important active moiety for antiviral activity. Virantmycin could be a suitable leading compound for an antiviral drug against PRV.
