Expression and significance of Ninj1, IL-1β and PGP-9.5 in ectopic endometrial tissues of patients with endometriosis

Abstract

Objective To investigate the expression levels of nerve injury-induced protein 1 (Ninjl), interleukin-1β(IL-1β), and protein gene product 9.5 (PGP-9.5) in the ectopic endometrial tissues of patients with endometriosis (EMT) and their relationship with dysmenorrhea. Methods Ectopic endometrial tissues from 40 patients with ovarian EMT diagnosed by post-ope-rative gynecological pathological examination in our hospital from April 2020 to April 2021 were selected as group A. In situ endo-metrial tissues from 20 of these patients were obtained as group B. Normal endometrial tissues from 20 patients with uterine fi-broids in the same period were collected as group C. Group A was divided into a dysmenorrhoea subgroup and a non-dysmenorrhoea subgroup according to the presence or absence of dysmenorrhoea, with 20 patients per subgroup. The expression levels of Ninj1, IL-1β, and PGP-9.5 in the endometrial tissues of groups A, B, and C were measured by immunohistochemistry, and their correlation was analyzed. The expression level of IL-1β in the peripheral blood of patiens in groups A and C was measured by enzyme-linked immunosorbent assay. Results The expression level of IL-1β in the peripheral blood of patients in group A was signifi-cantly higher than that in group C (t=-4.184,P<0.05). The positive expression rates of three proteins in the endometrial tissues were significantly increased in groups A and B compared with group C (χ2=4.286-19.394, P<0.05)and in group A compared with group B (χ2=4.149-5.272,P<0.05). The positive expression rates of Ninjl and PGP-9.5 proteins in the endometrial tissues were significantly higher in the dysmenorrhoea subgroupthan in the non-dysmenorrhoea subgroup (χ2=8.533,11.905,P<0.05). The expression of Ninj1 was positively correlated with the expression of PGP-9.5 and IL-1β in the endometrial tissues of group A (r=0.733, 0.628,P<0.05). Conclusion Ninjl, IL-1β, and PGP-9.5 are highly expressed in the ectopic endometrial tissues,which may be involved in the occurrence and development of EMT. The positive expression rates of both Ninjl and PGP-9.5 in the endometrial tissues of the dysmenorrhoea subgroup were higher than those in the non-dysmenorrhoea subgroup, which may be closely related to the mechanism of EMT pain.

Keywords

• endometriosis|cell adhesion molecules, neuronal|interleukin-1beta|nerve tissue proteins|dysmenorrhea