精准医学杂志 (Aug 2023)
EFFECTS OF Ang-1 MIMICKING PEPTIDE IN SYNERGY WITH VASCULAR ENDOTHELIAL GROWTH FACTOR ON CARDIAC FUNCTION IN RATS WITH MYOCARDIAL INFARCTION
Abstract
Objective To investigate the effects of cardiac extracellular matrix (c-ECM) combined with collagen-binding vascular endothelial growth factor (CBD-VEGF) and Ang-1 mimicking peptide (AMP) on cardiac function in rats with myocardial infarction (MI). Methods MTT assay was used to measure the activity of AMP in human umbilical vein endothelial cells (HUVECs) in vitro, and the binding of Biotin-modified AMP (Bio-AMP) to c-ECM was tested. A myocardial infarction model was established by ligation of the left anterior descending artery in SD rats. The rats were randomly divided into PBS group (group A), c-ECM/CBD-VEGF group (group B), and CBD-VEGF/c-ECM/AMP group (group C), with four rats in each group. The rats were treated by injection of PBS, CBD-VEGF/c-ECM, or CBD-VEGF/c-ECM/AMP at the infarct site. After 3 months of treatment, echocardiography was used to evaluate the cardiac function of rats in the three groups. Immunofluorescence staining was used to determine the number of blood vessels as well as the ratios of vWF positive area and ZO1 positive area in the myocardial infarction area. Results MTT assay showed that AMP and Ang-1 had similar ability to promote the proliferation of HUVECs. The binding experiment showed that Bio-AMP could be attached to c-ECM. After 3 months of treatment, the ejection fraction was significantly higher in group C than in group A and group B (t=7.794,3.613,P<0.05). Immunofluorescence staining showed that the number of blood vessels and the ratio of vWF positive area were significantly higher in group C than in group A (t=4.950,22.390,P<0.05), and the ratio of vWF positive area was significantly higher in group C than in group B (t=11.400,P<0.05). The ZO1 positive area ratio was significantly higher in group C and group B than in group A (t=13.290,4.328,P<0.05). The ZO1 positive area ratio was significantly higher in group C than in group B (t=8.243,P<0.05). Conclusion Injection of the CBD-VEGF/c-ECM/AMP hydrogel synthesized in this study at the myocardial infarction area can promote the recovery of cardiac function and enhance angiogenesis and cell connection in rats after myocardial infarction.
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