Exercise and Nano-curcumin supplementation mediates cross-talk between MAPK/ERK signaling in the regulation of inflammatory disease

Abstract

Impaired cell internal settings and excessive proliferation causes the occurrence of diverse ranges of syndrom and diseases. The pathological stress underlying these conditions triggers persistent flux through multiple intracellular signaling pathways amongst MAPK/ERK as master regulator. Regarding the anti-inflammatory effects of muscle contraction induced myokines and nano-curcumin supplementation, we aimed to investigate the effects of aerobic training and nano-curcumin supplementation on RAS and ERK gene expression in rat muscle tissue. In this experimental study, 32 male wistar rats (aged 4-6 weeks, 130-150 g) were randomly assigned into 4 groups, including Control (C), Moderate Intensity Continious Training (MICT), Nano-Curcumin Supplementation (NCS) and Moderate Intensity Continious Training + Nano-curcumin (MICT+NCS). The training groups implemented the MICT protocol consisted of running at a velocity of 18-20 m/min, 5 days a week and for a total time of 4 weeks. The Supplement groups received 80 mg/kg/day through oral gavage. Regarding the results of one-way ANOVA, 4 weeks of moderate intensity aerobic exercise and Nano-curcumin supplementation led to a significant difference in the RAS (P=0.001) and ERK (P=0.01) gene expression levels in muscle tissue of rats among the study groups. Also, the results of the Bonferronie test showed that implementation of 4 weeks of MICT along with nano-curcumin supplementation alleviated the RAS/ERK gene expression levels, meanwhile nano-curcumin more efficiently down-regulated the pathway; suggesting that nano-curcumin can be an effective ergogenic aid for improving anti-inflmmatory properties through RAS/ERK signaling pathway.

Keywords

• aerobic exercise
• nano-curcumin
• ras
• erk