Nature Communications (Oct 2018)

Enhanced validation of antibodies for research applications

  • Fredrik Edfors,
  • Andreas Hober,
  • Klas Linderbäck,
  • Gianluca Maddalo,
  • Alireza Azimi,
  • Åsa Sivertsson,
  • Hanna Tegel,
  • Sophia Hober,
  • Cristina Al-Khalili Szigyarto,
  • Linn Fagerberg,
  • Kalle von Feilitzen,
  • Per Oksvold,
  • Cecilia Lindskog,
  • Björn Forsström,
  • Mathias Uhlen

DOI
https://doi.org/10.1038/s41467-018-06642-y
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 10

Abstract

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Five validation pillars have been proposed to verify the specificity of research antibodies. Here the authors screen 6,000 antibodies from the Human Protein Atlas with these methods to provide an antibody validation resource for providers and users.