Large-scale perfused tissues via synthetic 3D soft microfluidics

Sergei Grebenyuk; Abdel Rahman Abdel Fattah; Manoj Kumar; Burak Toprakhisar; Gregorius Rustandi; Anja Vananroye; Idris Salmon; Catherine Verfaillie; Mark Grillo; Adrian Ranga

doi:10.1038/s41467-022-35619-1

Nature Communications (Jan 2023)

Large-scale perfused tissues via synthetic 3D soft microfluidics

Sergei Grebenyuk,
Abdel Rahman Abdel Fattah,
Manoj Kumar,
Burak Toprakhisar,
Gregorius Rustandi,
Anja Vananroye,
Idris Salmon,
Catherine Verfaillie,
Mark Grillo,
Adrian Ranga

Affiliations

Sergei Grebenyuk: Laboratory of Bioengineering and Morphogenesis, Biomechanics Section, Department of Mechanical Engineering, KU Leuven
Abdel Rahman Abdel Fattah: Laboratory of Bioengineering and Morphogenesis, Biomechanics Section, Department of Mechanical Engineering, KU Leuven
Manoj Kumar: Stem Cell Institute Leuven and Department of Development and Regeneration, Faculty of Medicine, KU Leuven
Burak Toprakhisar: Stem Cell Institute Leuven and Department of Development and Regeneration, Faculty of Medicine, KU Leuven
Gregorius Rustandi: Laboratory of Bioengineering and Morphogenesis, Biomechanics Section, Department of Mechanical Engineering, KU Leuven
Anja Vananroye: Laboratory of Soft Matter, Rheology and Technology, Department of Chemical Engineering, KU Leuven
Idris Salmon: Laboratory of Bioengineering and Morphogenesis, Biomechanics Section, Department of Mechanical Engineering, KU Leuven
Catherine Verfaillie: Stem Cell Institute Leuven and Department of Development and Regeneration, Faculty of Medicine, KU Leuven
Mark Grillo: Grillo Consulting Inc.
Adrian Ranga: Laboratory of Bioengineering and Morphogenesis, Biomechanics Section, Department of Mechanical Engineering, KU Leuven

DOI: https://doi.org/10.1038/s41467-022-35619-1
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 18

Abstract

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Bioengineering live tissues has remained challenging due to limited nutrient exchange in the growing tissues. Here, the authors have developed micro-perfused 2-photon printing of 3D microfluidics, to engineer large-scale, viable and functional neural and hepatic 3D tissues.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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