Gut Bifidobacterium pseudocatenulatum protects against fat deposition by enhancing secondary bile acid biosynthesis
Andong Zha,
Ming Qi,
Yuankun Deng,
Hao Li,
Nan Wang,
Chengming Wang,
Simeng Liao,
Dan Wan,
Xia Xiong,
Peng Liao,
Jing Wang,
Yulong Yin,
Bi'e Tan
Affiliations
Andong Zha
Key Laboratory of Hunan Province for the Products Quality Regulation of Livestock and Poultry College of Animal Science and Technology, Hunan Agricultural University Changsha China
Ming Qi
Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro‐Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha China
Yuankun Deng
Key Laboratory of Hunan Province for the Products Quality Regulation of Livestock and Poultry College of Animal Science and Technology, Hunan Agricultural University Changsha China
Hao Li
Key Laboratory of Hunan Province for the Products Quality Regulation of Livestock and Poultry College of Animal Science and Technology, Hunan Agricultural University Changsha China
Nan Wang
Key Laboratory of Hunan Province for the Products Quality Regulation of Livestock and Poultry College of Animal Science and Technology, Hunan Agricultural University Changsha China
Chengming Wang
Key Laboratory of Hunan Province for the Products Quality Regulation of Livestock and Poultry College of Animal Science and Technology, Hunan Agricultural University Changsha China
Simeng Liao
Key Laboratory of Hunan Province for the Products Quality Regulation of Livestock and Poultry College of Animal Science and Technology, Hunan Agricultural University Changsha China
Dan Wan
Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro‐Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha China
Xia Xiong
Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro‐Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha China
Peng Liao
Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro‐Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences Changsha China
Jing Wang
Key Laboratory of Hunan Province for the Products Quality Regulation of Livestock and Poultry College of Animal Science and Technology, Hunan Agricultural University Changsha China
Yulong Yin
Key Laboratory of Hunan Province for the Products Quality Regulation of Livestock and Poultry College of Animal Science and Technology, Hunan Agricultural University Changsha China
Bi'e Tan
Key Laboratory of Hunan Province for the Products Quality Regulation of Livestock and Poultry College of Animal Science and Technology, Hunan Agricultural University Changsha China
Abstract Gut microbiome is crucial for lipid metabolism in humans and animals. However, how specific gut microbiota and their associated metabolites impact fat deposition remains unclear. In this study, we demonstrated that the colonic microbiome of lean and obese pigs differentially contributes to fat deposition, as evidenced by colonic microbiota transplantation experiments. Notably, the higher abundance of Bifidobacterium pseudocatenulatum was significantly associated with lower backfat thickness in lean pigs. Microbial‐derived lithocholic acid (LCA) species were also significantly enriched in lean pigs and positively correlated with the abundance of B. pseudocatenulatum. In a high‐fat diet (HFD)‐fed mice model, administration of live B. pseudocatenulatum decreased fat deposition and enhances colonic secondary bile acid biosynthesis. Importantly, pharmacological inhibition of the bile salt hydrolase (BSH), which mediates secondary bile acid biosynthesis, impaired the anti‐fat deposition effect of B. pseudocatenulatum in antibiotic‐pretreated, HFD‐fed mice. Furthermore, dietary LCA also decreased fat deposition in HFD‐fed rats and obese pig models. These findings provide mechanistic insights into the anti‐fat deposition role of B. pseudocatenulatum and identify BSH as a potential target for preventing excessive fat deposition in humans and animals.
