Nature Communications (Dec 2022)

STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity

  • Hiroaki Ohishi,
  • Seiru Shimada,
  • Satoshi Uchino,
  • Jieru Li,
  • Yuko Sato,
  • Manabu Shintani,
  • Hitoshi Owada,
  • Yasuyuki Ohkawa,
  • Alexandros Pertsinidis,
  • Takashi Yamamoto,
  • Hiroshi Kimura,
  • Hiroshi Ochiai

DOI
https://doi.org/10.1038/s41467-022-35286-2
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 19

Abstract

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Using the newly developed STREAMING-tag system, the authors find that clusters of RNA polymerase II and BRD4 are formed specifically in the transcriptionally active state near the Nanog gene in mouse embryonic stem cells.