Nature Communications (Aug 2022)
Single-cell transcriptome and translatome dual-omics reveals potential mechanisms of human oocyte maturation
- Wenqi Hu,
- Haitao Zeng,
- Yanan Shi,
- Chuanchuan Zhou,
- Jiana Huang,
- Lei Jia,
- Siqi Xu,
- Xiaoyu Feng,
- Yanyan Zeng,
- Tuanlin Xiong,
- Wenze Huang,
- Peng Sun,
- Yajie Chang,
- Tingting Li,
- Cong Fang,
- Keliang Wu,
- Lingbo Cai,
- Wuhua Ni,
- Yan Li,
- Zhiyong Yang,
- Qiangfeng Cliff Zhang,
- RiCheng Chian,
- Zijiang Chen,
- Xiaoyan Liang,
- Kehkooi Kee
Affiliations
- Wenqi Hu
- Center for Stem Cell Biology and Regenerative Medicine, Department of Basic Medical Sciences, School of Medicine, Tsinghua University
- Haitao Zeng
- Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University
- Yanan Shi
- Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University
- Chuanchuan Zhou
- Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University
- Jiana Huang
- Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University
- Lei Jia
- Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University
- Siqi Xu
- Center for Stem Cell Biology and Regenerative Medicine, Department of Basic Medical Sciences, School of Medicine, Tsinghua University
- Xiaoyu Feng
- Center for Stem Cell Biology and Regenerative Medicine, Department of Basic Medical Sciences, School of Medicine, Tsinghua University
- Yanyan Zeng
- Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University
- Tuanlin Xiong
- MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology and Frontier Research Center for Biological Structure, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University
- Wenze Huang
- MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology and Frontier Research Center for Biological Structure, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University
- Peng Sun
- Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University
- Yajie Chang
- Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University
- Tingting Li
- Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University
- Cong Fang
- Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University
- Keliang Wu
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University
- Lingbo Cai
- State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University
- Wuhua Ni
- Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University
- Yan Li
- Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University
- Zhiyong Yang
- Center for Reproductive Medicine, Shanghai Tenth People’s Hospital of Tongji University
- Qiangfeng Cliff Zhang
- MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology and Frontier Research Center for Biological Structure, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University
- RiCheng Chian
- Center for Reproductive Medicine, Shanghai Tenth People’s Hospital of Tongji University
- Zijiang Chen
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University
- Xiaoyan Liang
- Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University
- Kehkooi Kee
- Center for Stem Cell Biology and Regenerative Medicine, Department of Basic Medical Sciences, School of Medicine, Tsinghua University
- DOI
- https://doi.org/10.1038/s41467-022-32791-2
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 17
Abstract
Development of methods for simultaneous single cell analysis of transcription and translation is still underway. Here, Hu et al. develop single-cell transcriptome and translatome dual-omics on human oocytes, which enables them to identify OOSP2 as an induction factor during human oocyte maturation.
