Cycles of protein condensation and discharge in nuclear organelles studied by fluorescence lifetime imaging

Artem Pliss; Svitlana M. Levchenko; Lixin Liu; Xiao Peng; Tymish Y. Ohulchanskyy; Indrajit Roy; Andrey N. Kuzmin; Junle Qu; Paras N. Prasad

doi:10.1038/s41467-019-08354-3

Nature Communications (Jan 2019)

Cycles of protein condensation and discharge in nuclear organelles studied by fluorescence lifetime imaging

Artem Pliss,
Svitlana M. Levchenko,
Lixin Liu,
Xiao Peng,
Tymish Y. Ohulchanskyy,
Indrajit Roy,
Andrey N. Kuzmin,
Junle Qu,
Paras N. Prasad

Affiliations

Artem Pliss: Institute for Lasers, Photonics and Biophotonics and Department of Chemistry, State University of New York
Svitlana M. Levchenko: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University
Lixin Liu: School of Physics and Optoelectronic Engineering, Xidian University
Xiao Peng: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University
Tymish Y. Ohulchanskyy: Institute for Lasers, Photonics and Biophotonics and Department of Chemistry, State University of New York
Indrajit Roy: Department of Chemistry, University of Delhi
Andrey N. Kuzmin: Institute for Lasers, Photonics and Biophotonics and Department of Chemistry, State University of New York
Junle Qu: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University
Paras N. Prasad: Institute for Lasers, Photonics and Biophotonics and Department of Chemistry, State University of New York

DOI: https://doi.org/10.1038/s41467-019-08354-3
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 9

Abstract

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Studying the condensation of proteins into membraneless organelles in live cells is highly challenging. Here the authors develop a fluorescence lifetime imaging approach to monitor the condensation of proteins in nuclear organelles and report coordinated and cyclic changes in several nuclear organelles.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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