Metabolic Pathway of Monounsaturated Lipids Revealed by In-Depth Structural Lipidomics by Mass Spectrometry
Simin Cheng,
Donghui Zhang,
Jiaxin Feng,
Qingyuan Hu,
Aolei Tan,
Zhuoning Xie,
Qinhua Chen,
Huimin Huang,
Ying Wei,
Zheng Ouyang,
Xiaoxiao Ma
Affiliations
Simin Cheng
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument,
Tsinghua University, Beijing 100084, China.
Donghui Zhang
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument,
Tsinghua University, Beijing 100084, China.
Jiaxin Feng
Department of Chemistry,
Tsinghua University, Beijing 100084, China.
Qingyuan Hu
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument,
Tsinghua University, Beijing 100084, China.
Aolei Tan
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument,
Tsinghua University, Beijing 100084, China.
Zhuoning Xie
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument,
Tsinghua University, Beijing 100084, China.
Sinopharm Dongfeng General Hospital,
Hubei University of Medicine, Experiment center of medicine, Shiyan, Hubei 442008, China.
Ying Wei
Sinopharm Dongfeng General Hospital,
Hubei University of Medicine, Experiment center of medicine, Shiyan, Hubei 442008, China.
Zheng Ouyang
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument,
Tsinghua University, Beijing 100084, China.
Xiaoxiao Ma
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument,
Tsinghua University, Beijing 100084, China.
The study of lipid metabolism relies on the characterization of the lipidome, which is quite complex due to the structure variations of the lipid species. New analytical tools have been developed recently for characterizing fine structures of lipids, with C=C location identification as one of the major improvements. In this study, we studied the lipid metabolism reprograming by analyzing glycerol phospholipid compositions in breast cancer cell lines with structural specification extended to the C=C location level. Inhibition of the lipid desaturase, stearoyl-CoA desaturase 1, increased the proportion of n-10 isomers that are produced via an alternative fatty acid desaturase 2 pathway. However, there were different variations of the ratio of n-9/n-7 isomers in C18:1-containing glycerol phospholipids after stearoyl-CoA desaturase 1 inhibition, showing increased tendency in MCF-7 cells, MDA-MB-468 cells, and BT-474 cells, but decreased tendency in MDA-MB-231 cells. No consistent change of the ratio of n-9/n-7 isomers was observed in SK-BR-3 cells. This type of heterogeneity in reprogrammed lipid metabolism can be rationalized by considering both lipid desaturation and fatty acid oxidation, highlighting the critical roles of comprehensive lipid analysis in both fundamental and biomedical applications.
