From July 2016 to September 2017, samples of fleshy fruits (38 samples, 50.93 kg) were collected in the municipalities of Rio Branco and Senador Guiomard, in the state of Acre, Brazil, aiming to expand our knowledge about the diversity of fruit flies, their host plants and associated parasitoids. In this work, we report the first record of Anastrepha serpentina (Wiedemann) and the parasitoid Asobara anastrephae (Muesebeck) in Acre. In addition, Pouteria caimito (Ruiz & Pav.) Radlk. (Sapotaceae) was found to be a new host for Ceratitis capitata in the Brazilian Amazon.
