Acta Biologica Sibirica (Dec 2017)
Thermophilic facultative anaerobic bacteria of the genus Geobacillus from bottom sediments of Lake Baikal
Abstract
Pure culture of thermophilic facultative anaerobic microorganism was isolated from surface sediment of methane seepage Posolsk Bank. New strain Geobacillus sp. PB15/Grf7geo does not differ from the typical strains by morphological and physiological-biochemical properties. It is able to withstand of wide temperature range with optimum growth between 55–60 °C. Chemoorganotroph. Phylogenetic analysis of the 16S rRNA sequence of the strain showed 97 % identity to cultivated strains of this genus. The strain forms a common clade with uncultivated representatives and does not cluster with sequences of cultivated strains within the genus Geobacillus in the phylogenetic tree. Strain Geobacillus sp. PB15/Grf7geo has been deposited to the collection of the VKM (=VKM B–3150T).
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