Cell Reports (Dec 2014)
Mycobacterium Tuberculosis Proteome Microarray for Global Studies of Protein Function and Immunogenicity
- Jiaoyu Deng,
- Lijun Bi,
- Lin Zhou,
- Shu-juan Guo,
- Joy Fleming,
- He-wei Jiang,
- Ying Zhou,
- Jia Gu,
- Qiu Zhong,
- Zong-xiu Wang,
- Zhonghui Liu,
- Rui-ping Deng,
- Jing Gao,
- Tao Chen,
- Wenjuan Li,
- Jing-fang Wang,
- Xude Wang,
- Haicheng Li,
- Feng Ge,
- Guofeng Zhu,
- Hai-nan Zhang,
- Jing Gu,
- Fan-lin Wu,
- Zhiping Zhang,
- Dianbing Wang,
- Haiying Hang,
- Yang Li,
- Li Cheng,
- Xiang He,
- Sheng-ce Tao,
- Xian-En Zhang
Affiliations
- Jiaoyu Deng
- State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
- Lijun Bi
- National Key Laboratory of Biomacromolecules, Key Laboratory of Non-Coding RNA and Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
- Lin Zhou
- Center for Tuberculosis Control of Guangdong Province, Guangzhou 510630, China
- Shu-juan Guo
- Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education); State Key Laboratory of Oncogenes and Related Genes; and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
- Joy Fleming
- National Key Laboratory of Biomacromolecules, Key Laboratory of Non-Coding RNA and Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
- He-wei Jiang
- Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education); State Key Laboratory of Oncogenes and Related Genes; and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
- Ying Zhou
- National Key Laboratory of Biomacromolecules, Key Laboratory of Non-Coding RNA and Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
- Jia Gu
- State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
- Qiu Zhong
- Center for Tuberculosis Control of Guangdong Province, Guangzhou 510630, China
- Zong-xiu Wang
- Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education); State Key Laboratory of Oncogenes and Related Genes; and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
- Zhonghui Liu
- National Key Laboratory of Biomacromolecules, Key Laboratory of Non-Coding RNA and Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
- Rui-ping Deng
- Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education); State Key Laboratory of Oncogenes and Related Genes; and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
- Jing Gao
- State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
- Tao Chen
- Center for Tuberculosis Control of Guangdong Province, Guangzhou 510630, China
- Wenjuan Li
- National Key Laboratory of Biomacromolecules, Key Laboratory of Non-Coding RNA and Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
- Jing-fang Wang
- Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education); State Key Laboratory of Oncogenes and Related Genes; and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
- Xude Wang
- State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
- Haicheng Li
- Center for Tuberculosis Control of Guangdong Province, Guangzhou 510630, China
- Feng Ge
- Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430071, China
- Guofeng Zhu
- Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China
- Hai-nan Zhang
- Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education); State Key Laboratory of Oncogenes and Related Genes; and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
- Jing Gu
- State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
- Fan-lin Wu
- Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education); State Key Laboratory of Oncogenes and Related Genes; and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
- Zhiping Zhang
- State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
- Dianbing Wang
- National Key Laboratory of Biomacromolecules, Key Laboratory of Non-Coding RNA and Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
- Haiying Hang
- National Key Laboratory of Biomacromolecules, Key Laboratory of Non-Coding RNA and Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
- Yang Li
- Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education); State Key Laboratory of Oncogenes and Related Genes; and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
- Li Cheng
- Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education); State Key Laboratory of Oncogenes and Related Genes; and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
- Xiang He
- Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education); State Key Laboratory of Oncogenes and Related Genes; and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
- Sheng-ce Tao
- Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education); State Key Laboratory of Oncogenes and Related Genes; and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
- Xian-En Zhang
- National Key Laboratory of Biomacromolecules, Key Laboratory of Non-Coding RNA and Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
- DOI
- https://doi.org/10.1016/j.celrep.2014.11.023
- Journal volume & issue
-
Vol. 9,
no. 6
pp. 2317 – 2329
Abstract
Poor understanding of the basic biology of Mycobacterium tuberculosis (MTB), the etiological agent of tuberculosis, hampers development of much-needed drugs, vaccines, and diagnostic tests. Better experimental tools are needed to expedite investigations of this pathogen at the systems level. Here, we present a functional MTB proteome microarray covering most of the proteome and an ORFome library. We demonstrate the broad applicability of the microarray by investigating global protein-protein interactions, small-molecule-protein binding, and serum biomarker discovery, identifying 59 PknG-interacting proteins, 30 bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) binding proteins, and 14 MTB proteins that together differentiate between tuberculosis (TB) patients with active disease and recovered individuals. Results suggest that the MTB rhamnose pathway is likely regulated by both the serine/threonine kinase PknG and c-di-GMP. This resource has the potential to generate a greater understanding of key biological processes in the pathogenesis of tuberculosis, possibly leading to more effective therapies for the treatment of this ancient disease.
