Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels

Gabriela Pacheco de Almeida Braga; Karina Sampaio Caiaffa; Rafaela Laruzo Rabelo; Vanessa Rodrigues dos Santos; Amanda Caselato Andolfatto Souza; Lucas da Silva Ribeiro; Emerson Rodrigues de Camargo; Anuradha Prakki; Cristiane Duque

doi:10.3390/jfb14030139

Journal of Functional Biomaterials (Mar 2023)

Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels

Gabriela Pacheco de Almeida Braga,
Karina Sampaio Caiaffa,
Rafaela Laruzo Rabelo,
Vanessa Rodrigues dos Santos,
Amanda Caselato Andolfatto Souza,
Lucas da Silva Ribeiro,
Emerson Rodrigues de Camargo,
Anuradha Prakki,
Cristiane Duque

Affiliations

Gabriela Pacheco de Almeida Braga: Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil
Karina Sampaio Caiaffa: Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil
Rafaela Laruzo Rabelo: Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil
Vanessa Rodrigues dos Santos: Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil
Amanda Caselato Andolfatto Souza: Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil
Lucas da Silva Ribeiro: Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos 13565-905, SP, Brazil
Emerson Rodrigues de Camargo: Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos 13565-905, SP, Brazil
Anuradha Prakki: Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada
Cristiane Duque: Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil

DOI: https://doi.org/10.3390/jfb14030139
Journal volume & issue: Vol. 14, no. 3
p. 139

Abstract

Read online

This study aimed to evaluate the effects of flavonoids incorporated into poly(N-vinylcaprolactam) (PNVCL) hydrogel on cell viability and mineralization markers of odontoblast-like cells. MDPC-23 cells were exposed to ampelopsin (AMP), isoquercitrin (ISO), rutin (RUT) and control calcium hydroxide (CH) for evaluation of cell viability, total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition by colorimetric assays. Based on an initial screening, AMP and CH were loaded into PNVCL hydrogels and had their cytotoxicity and effect on mineralization markers determined. Cell viability was above 70% when MDPC-23 cells were treated with AMP, ISO and RUT. AMP showed the highest ALP activity and mineralized nodule deposition. Extracts of PNVCL+AMP and PNVCL+CH in culture medium (at the dilutions of 1/16 and 1/32) did not affect cell viability and stimulated ALP activity and mineralized nodules’ deposition, which were statistically higher than the control in osteogenic medium. In conclusion, AMP and AMP-loaded PNVCL hydrogels were cytocompatible and able to induce bio-mineralization markers in odontoblast-cells.

Published in Journal of Functional Biomaterials

ISSN: 2079-4983 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology; Medicine: Medicine (General)
Website: http://www.mdpi.com/journal/jfb/

About the journal

Abstract

Keywords