Genes (May 2023)
First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of <i>Trichopoda pennipes</i>, a Parasitoid of <i>Nezara viridula</i>
- Mesfin Bogale,
- Shova Mishra,
- Kendall Stacey,
- Lillie Rooney,
- Paula Barreto,
- Gina Bishop,
- Katherine Bossert,
- Kalista Bremer,
- Daniel Bustamante,
- Lila Chan,
- Quan Chau,
- Julian Cordo,
- Alyssa Diaz,
- Jordan Hacker,
- Lily Hadaegh,
- Taryn Hibshman,
- Kimberly Lastra,
- Fion Lee,
- Alexandra Mattia,
- Bao Nguyen,
- Gretchen Overton,
- Victoria Reis,
- Daniel Rhodes,
- Emily Roeder,
- Muhamed Rush,
- Oscar Salichs,
- Mateo Seslija,
- Nicholas Stylianou,
- Vivek Vemugunta,
- Min Yun,
- Anthony Auletta,
- Norman Leppla,
- Peter DiGennaro
Affiliations
- Mesfin Bogale
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Shova Mishra
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Kendall Stacey
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Lillie Rooney
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Paula Barreto
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Gina Bishop
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Katherine Bossert
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Kalista Bremer
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Daniel Bustamante
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Lila Chan
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Quan Chau
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Julian Cordo
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Alyssa Diaz
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Jordan Hacker
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Lily Hadaegh
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Taryn Hibshman
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Kimberly Lastra
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Fion Lee
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Alexandra Mattia
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Bao Nguyen
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Gretchen Overton
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Victoria Reis
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Daniel Rhodes
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Emily Roeder
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Muhamed Rush
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Oscar Salichs
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Mateo Seslija
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Nicholas Stylianou
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Vivek Vemugunta
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Min Yun
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Anthony Auletta
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Norman Leppla
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- Peter DiGennaro
- Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, USA
- DOI
- https://doi.org/10.3390/genes14061172
- Journal volume & issue
-
Vol. 14,
no. 6
p. 1172
Abstract
Trichopoda pennipes is a tachinid parasitoid of several significant heteropteran agricultural pests, including the southern green stink bug, Nezara viridula, and leaf-footed bug, Leptoglossus phyllopus. To be used successfully as a biological control agent, the fly must selectively parasitize the target host species. Differences in the host preference of T. pennipes were assessed by assembling the nuclear and mitochondrial genomes of 38 flies reared from field-collected N. viridula and L. phyllopus. High-quality de novo draft genomes of T. pennipes were assembled using long-read sequencing. The assembly totaled 672 MB distributed among 561 contigs, having an N50 of 11.9 MB and a GC of 31.7%, with the longest contig at 28 MB. The genome was assessed for completeness using BUSCO in the Insecta dataset, resulting in a score of 99.4%, and 97.4% of the genes were single copy-loci. The mitochondrial genomes of the 38 T. pennipes flies were sequenced and compared to identify possible host-determined sibling species. The assembled circular genomes ranged from 15,345 bp to 16,390 bp and encode 22 tRNAs, two rRNAs, and 13 protein-coding genes (PCGs). There were no differences in the architecture of these genomes. Phylogenetic analyses using sequence information from 13 PCGs and the two rRNAs individually or as a combined dataset resolved the parasitoids into two distinct lineages: T. pennipes that parasitized both N. viridula and L. phyllopus, and others that parasitized only L. phyllopus.
Keywords
- <i>Trichopoda pennipes</i>
- <i>Nezara viridula</i>
- <i>Leptoglossus phyllopus</i>
- mitogenome
- genome
- sibling species
