Application of molecular tools in detecting zoonotic pathogens in organic fertilizers and liquid supplements

Abstract

The use of organic fertilizers and liquid supplements for crop production is rapidly growing as an alternative system to conventional agriculture. However, very little is known about the public health issues related to pathogens. This study endeavors to identify the important zoonotic pathogens with the current molecular diagnostic tools, Loop-mediated isothermal amplification (LAMP) and Recombinase polymerase amplification (RPA), against the conventional pathogen detection. These cost-effective molecular techniques have proven to be confirmatory tests of the target pathogens present in organic fertilizers and liquid supplements, which recommends an advancement for the comprehensive field surveillance-response approach in many developing countries with resource-limited settings quality assurance and safety implementation of organic biosolids for sustainable agricultural farming.

Keywords

• Organic fertilizers
• Liquid supplements
• Polymerase chain reaction (PCR)
• Loop-mediated isothermal amplification (LAMP)
• Recombinase polymerase amplification (RPA)