Simultaneous Nitrification and Denitrification under Aerobic Atmosphere by Newly Isolated <i>Pseudomona aeruginosa</i> LS82
Jun Luo,
Yating Jia,
Yuancai Chen,
Chen Chen,
Fernada Leite Lobo,
Janine Brandao de Farias Mesquita,
Asheesh Kumar Yadav,
Shan Huang
Affiliations
Jun Luo
State Environmental Protection Key Laboratory of Urban Ecological Environment Simulation and Protection, South China Institute of Environmental Sciences, Ministry of Ecology and Environment of China, Guangzhou 510535, China
Yating Jia
Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, School of Environment and Energy, South China University of Technology, Guangzhou 510006, China
Yuancai Chen
Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, School of Environment and Energy, South China University of Technology, Guangzhou 510006, China
Chen Chen
State Environmental Protection Key Laboratory of Urban Ecological Environment Simulation and Protection, South China Institute of Environmental Sciences, Ministry of Ecology and Environment of China, Guangzhou 510535, China
Fernada Leite Lobo
Departamento de Engenharia Hidraulica e Ambiental, Universidade Federal do Ceara, Fortaleza 60440-554, Brazil
Janine Brandao de Farias Mesquita
Crateús Campus, Federal University of Ceará, Crateús 63707-800, Brazil
Asheesh Kumar Yadav
CSIR-Institute of Minerals and Materials Technology, Bhubaneswar 75113, India
Shan Huang
Department of Civil and Environmental Engineering, Princeton University, Princeton, NJ 08540, USA
Discharge of wastewater contained high amount of nitrogen would cause eutrophication to water bodies. Simultaneous nitrification and denitrification (SND) has been confirmed as an effective process, the isolation of SND bacteria is crucial for its successful operation. In this study, an SND strain was isolated and identified as Pseudomona aeruginosa LS82, which exhibited a rapid growth rate (0.385 h−1) and good nitrogen removal performance (4.96 mg N·L−1·h−1). Response surface methodology was applied to optimize the TN removal conditions, at which nearly complete nitrogen (99.8 ± 0.9%) removal were obtained within 18 h at the condition: pH 8.47, 100 rpm and the C/N ratio of 19.7. The saddle-shaped contours confirmed that the interaction of pH and inoculum size would influence the removal of total nitrogen significantly. Kinetic analyses indicated that the reduction of nitrite was the rate-limiting step in the SND process. Our research suggested strain LS82 can serve as a promising candidate for the treatment of ammonium rich wastewater, and expended our understanding the nitrogen removal mechanism in the SND process.
