Immunomodulatory efficacy of methanolic extract of Ajuga macrosperma in cyclophosphamide induced immunocompromised rat

Abstract

The present investigation was undertaken to evaluate immunomodulatory potential of Ajuga macrosperma against cyclophoshphamide (CP) induced immunosupression in rats. The immunological parameters such as haemaglutation (HA) titre and serum immunoglobulin (Ig) for humoral immunity (HI); lymphocyte stimulation test (LST), phagocytic index test, neutrophil adhesion test (NAT) and delayed type hypersensitivity reaction (DTH) for cellular immunity(CMI) were determined. The cyclophoshphamide treatment significantly suppressed both HI and CMI responses, which were significantly improved following treatment with 100 ppm and 200 ppm of methanolic extract of Ajuga macrosperma (MEAM) in dose dependent manner. The decreases in HI and CMI related parameters by CP treatment revealed immunosuppresion, whereas simultaneous treatment with MEAM ameliorated immune response which indicated immunomodulatory properties of Ajuga macrosperma.

Keywords

• Ajuga macrosperma
• Cyclophosphamide
• Immunomodulation
• Rat