Triamcinolone suppresses retinal vascular pathology via a potent interruption of proinflammatory signal-regulated activation of VEGF during a relative hypoxia

Y.H. Kim; I.Y. Chung; M.Y. Choi; Y.S. Kim; J.H. Lee; C.H. Park; S.S. Kang; G.S. Roh; W.S. Choi; J.M. Yoo; G.J. Cho

Neurobiology of Disease (Jun 2007)

Triamcinolone suppresses retinal vascular pathology via a potent interruption of proinflammatory signal-regulated activation of VEGF during a relative hypoxia

Y.H. Kim,
I.Y. Chung,
M.Y. Choi,
Y.S. Kim,
J.H. Lee,
C.H. Park,
S.S. Kang,
G.S. Roh,
W.S. Choi,
J.M. Yoo,
G.J. Cho

Affiliations

Y.H. Kim: Department of Anatomy and Neurobiology, College of Medicine, Gyeongsang National University, Chilam-dong 92, Jinju, Gyungnam 660-751, South Korea
I.Y. Chung: Department of Ophthalmology, Institute of Health Science, College of Medicine, Gyeongsang National University, Jinju, Gyeongnam 660-751, South Korea
M.Y. Choi: Department of Anatomy and Neurobiology, College of Medicine, Gyeongsang National University, Chilam-dong 92, Jinju, Gyungnam 660-751, South Korea
Y.S. Kim: Department of Anatomy and Neurobiology, College of Medicine, Gyeongsang National University, Chilam-dong 92, Jinju, Gyungnam 660-751, South Korea
J.H. Lee: Department of Anatomy and Neurobiology, College of Medicine, Gyeongsang National University, Chilam-dong 92, Jinju, Gyungnam 660-751, South Korea
C.H. Park: Department of Anatomy and Neurobiology, College of Medicine, Gyeongsang National University, Chilam-dong 92, Jinju, Gyungnam 660-751, South Korea
S.S. Kang: Department of Anatomy and Neurobiology, College of Medicine, Gyeongsang National University, Chilam-dong 92, Jinju, Gyungnam 660-751, South Korea
G.S. Roh: Department of Anatomy and Neurobiology, College of Medicine, Gyeongsang National University, Chilam-dong 92, Jinju, Gyungnam 660-751, South Korea
W.S. Choi: Department of Anatomy and Neurobiology, College of Medicine, Gyeongsang National University, Chilam-dong 92, Jinju, Gyungnam 660-751, South Korea
J.M. Yoo: Department of Ophthalmology, Institute of Health Science, College of Medicine, Gyeongsang National University, Jinju, Gyeongnam 660-751, South Korea
G.J. Cho: Department of Anatomy and Neurobiology, College of Medicine, Gyeongsang National University, Chilam-dong 92, Jinju, Gyungnam 660-751, South Korea; Corresponding author. Fax: +82 55 761 3398.

Journal volume & issue: Vol. 26, no. 3
pp. 569 – 576

Abstract

Read online

We examined the effect of triamcinolone acetonide (TA), a corticosteroid, on the relationship between vascular pathophysiology and vascular endothelial growth factor (VEGF) activation in the retina of a rat model of oxygen-induced retinopathy (OIR). OIR was induced by exposure of hyperoxia (80% oxygen) to Sprague–Dawley (SD) rats from P2 to P14 and then returned to normoxic conditions. TA was intravitreal-injected once into the right eye of OIR rats at P15. Effects of TA on vascular pathophysiology or changes of various genes in response to hypoxia and/or proinflammation under hypoxic retina were assessed by the Evans-blue method, fluorescein isothiocyanate-dextran (FITC-D) infusion, immunoblotting, and ELIZA. TA not only reduced retinal neovascularization and vascular leakage in the OIR-rat retina, but also blocked the induction of hypoxia-response proinflammatory genes before it negatively controlled VEGF activation. These findings suggest a potential that TA suppresses retinal neovascular pathophysiology via proinflammation-mediated activation of VEGF during hypoxia.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

About the journal

Abstract

Keywords