Construction of transgenic swine with induced expression of Cre recombinase

L. Chen; L. Li; D. Pang; Z. Li; T. Wang; M. Zhang; N. Song; S. Yan; L.X. Lai; H. Ouyang

Animal (Jan 2010)

Construction of transgenic swine with induced expression of Cre recombinase

L. Chen,
L. Li,
D. Pang,
Z. Li,
T. Wang,
M. Zhang,
N. Song,
S. Yan,
L.X. Lai,
H. Ouyang

Affiliations

L. Chen: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; The Basis of the Faculty of Medicine, Department of BeiHua University, Jilin 132013, PR China
L. Li: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
D. Pang: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
Z. Li: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
T. Wang: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
M. Zhang: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
N. Song: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
S. Yan: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
L.X. Lai: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
H. Ouyang: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China

Journal volume & issue: Vol. 4, no. 5
pp. 767 – 771

Abstract

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Miniature pigs have been recognized as valuable experimental animals in medical research. However, porcine models related to gene knockout of human diseases are not widely available. The objective of this study was to establish Mx1-Cre pigs using somatic cell nuclear transfer. In this study, we created transgenic pigs using somatic cell nuclear transfer (SCNT). Transfer of 210, 230, 250 and 215 zygotes to four surrogates produced 10 piglets. The Cre recombinase expression in transgenic pigs was studied using reverse transcriptase (RT)-PCR and immunohistochemistry. Mx1-Cre swine were shown to harbor the Cre gene in their genomic DNA using the PCR. In conclusion, Mx1-Cre transgenic piglets were successfully produced by SCNT. These transgenic swine, in conjunction with inducible systems for controlling Cre expression and function, are likely to have a profound impact on the study of human diseases.

Cre recombinase, somatic cell nuclear transfer (SCNT), pig

Published in Animal

ISSN: 1751-7311 (Print); 1751-732X (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/animal/

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