Construction of transgenic swine with induced expression of Cre recombinase
L. Chen,
L. Li,
D. Pang,
Z. Li,
T. Wang,
M. Zhang,
N. Song,
S. Yan,
L.X. Lai,
H. Ouyang
Affiliations
L. Chen
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; The Basis of the Faculty of Medicine, Department of BeiHua University, Jilin 132013, PR China
L. Li
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
D. Pang
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
Z. Li
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
T. Wang
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
M. Zhang
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
N. Song
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
S. Yan
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
L.X. Lai
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
H. Ouyang
Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China; College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, PR China
Miniature pigs have been recognized as valuable experimental animals in medical research. However, porcine models related to gene knockout of human diseases are not widely available. The objective of this study was to establish Mx1-Cre pigs using somatic cell nuclear transfer. In this study, we created transgenic pigs using somatic cell nuclear transfer (SCNT). Transfer of 210, 230, 250 and 215 zygotes to four surrogates produced 10 piglets. The Cre recombinase expression in transgenic pigs was studied using reverse transcriptase (RT)-PCR and immunohistochemistry. Mx1-Cre swine were shown to harbor the Cre gene in their genomic DNA using the PCR. In conclusion, Mx1-Cre transgenic piglets were successfully produced by SCNT. These transgenic swine, in conjunction with inducible systems for controlling Cre expression and function, are likely to have a profound impact on the study of human diseases.
