Biodegradation of low-density polyethylene LDPE by marine bacterial strains Gordonia alkanivorans PBM1 and PSW1 isolated from Mediterranean Sea, Alexandria, Egypt

Abstract

Abstract Plastic has become an essential part of daily human activity. Nonetheless, its over-utilization has resulted in environmental accumulation, leading to marine contamination. Biodegradation is the most effective approach for managing synthetic plastic waste. It encompasses various biological processes that depolymerize polymeric compounds into oligomers or monomers that can enter the biogeochemical cycle. Although research on microplastic biodegradation is abundant and increasing, studies on the biodegradation of low-density polyethylene (LDPE) by marine microorganisms remain scarce and underexplored. In the present study, a total of 17 bacterial isolates were isolated from plastic-contaminated sites in Abu Qir Bay, Alexandria, Egypt. Two bacterial strains demonstrated the highest LDPE biodegradation potential and were identified using 16 S rRNA sequencing, exhibiting 100% and 99.87% sequence identity to Gordonia alkanivorans. Biodegradation of LDPE was confirmed through dry weight loss, with G. alkanivorans strains PSW1 and PBM1 achieving reductions of 0.88 ± 0.658% and 0.66 ± 0.508%, respectively. Biodegradation was further confirmed through the formation of cracks and cavities observed through scanning electron microscopy (SEM). Infrared analysis indicated significant changes in LDPE functional groups and a decrease in the carbonyl index. Biodegradation of LDPE films was also demonstrated through gas chromatography-mass spectrometry (GC/MS) via the release of metabolites, correlating with LDPE utilization. The findings highlight the ability of marine bacteria G. alkanivorans strains PSW1 and PBM1 to biodegrade LDPE.

Keywords

• Marine
• Plastics
• LDPE
• Bacteria
• Biodegradation