Supportive data on the regulation of GLUT4 activity by 3-O-methyl-D-glucose
Ofer Shamni,
Guy Cohen,
Arie Gruzman,
Hilal Zaid,
Amira Klip,
Erol Cerasi,
Shlomo Sasson
Affiliations
Ofer Shamni
The Institute for Drug Research, Section of Pharmacology, Diabetes Research Unit, Faculty of Medicine, The Hebrew University, Jerusalem 9112102, Israel
Guy Cohen
The Institute for Drug Research, Section of Pharmacology, Diabetes Research Unit, Faculty of Medicine, The Hebrew University, Jerusalem 9112102, Israel
Arie Gruzman
The Institute for Drug Research, Section of Pharmacology, Diabetes Research Unit, Faculty of Medicine, The Hebrew University, Jerusalem 9112102, Israel
Hilal Zaid
Program in Cell Biology, Hospital for Sick Children, Toronto, OT, Canada M5G 1XB
Amira Klip
Program in Cell Biology, Hospital for Sick Children, Toronto, OT, Canada M5G 1XB
Erol Cerasi
Endocrinology and Metabolism Service, Department of Internal Medicine, The Hebrew University-Hadassah Medical Center, Jerusalem 9112001, Israel
Shlomo Sasson
The Institute for Drug Research, Section of Pharmacology, Diabetes Research Unit, Faculty of Medicine, The Hebrew University, Jerusalem 9112102, Israel
The data presented in this article are related to the research article entitled “Regulation of GLUT4 activity in myotubes by 3-O-methyl-D-glucose” (Shamni et al., 2017) [1]. These data show that the experimental procedures used to analyze the effects of 3-O-methyl-D-glucose (MeGlc) on the rate of hexose transport into myotubes were valid and controlled. The stimulatory effect of MeGlc was limited to glucose transporter 4 (GLUT4) and was independent of ambient glucose and protein synthesis. Cornish-Bowden kinetic analysis of uptake data revealed that MeGlc attenuated indinavir-induced inhibition of hexose transport in a competitive manner.
