Robust ordered mRNA differential display: an improved method for global gene expression profiling

Abstract

Global gene expression profiling (GGEP) plays a pivotal role in biological research. We developed an improved GGEP method called “robust ordered mRNA differential display (RoDD)” by combining mRNA differential display (DD) and complementary DNA amplified fragment length polymorphisms (cDNA-AFLP) using elaborately designed primers and a poly (dT:A) replacement technique. Redundancy was minimized by bead-based isolation and coverage was improved by using restriction enzymes that recognized 4-bp sites. This method offers the common virtues of gel-based methods along with the reliability of cDNA-AFLP. The most significant advantage of RoDD over current gel-based methods is greatly improved coverage and minimized redundancy.global gene expression profiling; mRNA differential display; complementary DNA amplified fragment length polymorphisms

Keywords

• global gene expression profiling
• mRNA differential display
• complementary DNA amplified fragment length polymorphisms