Bacteriological profile and antimicrobial susceptibility pattern of pyogenic wound infections at a tertiary care hospital

Abstract

Introduction: Infection of the skin and soft tissue due to injury, surgery, or burns may result in the formation of allexudates. Dead leucocytes, cellular debris and necrotic tissue are responsible for formation of exudates. Relentless exposure to devitalized tissue associated with a slow-healing chronic wound is likely to promote the colonisation and establishment of a wide range of bacteria. Mixed populations of both aerobic and anaerobic microorganisms are accountable for most acute and chronic wound infections. Therefore this study was carried out to investigate the aerobic bacterial isolates responsible for pyogenic wound infections and their antimicrobial susceptibility pattern for clinical management. Material and Methods: Pus samples were collected with sterile disposable cotton swabs and pus aspirates in syringes from suspected patients of pyogenic wound infections. They were processed using standard microbiological techniques and identification of isolate from positive cultures was done using conventional biochemical test. The antibiotic sensitivity testing of all isolates was performed by Kirby Bauer disc diffusion method on Muller Hinton agar and interpreted as per CLSI guidelines. Results: In our study out of 288 samples, 192(66.66%) were culture positive isolates and 96 (33.33%) were culture negative isolates. Out of 192 culture positive isolates, 76(39.58%) were Gram positive isolates and 116(60.41%) were Gram negative isolates. Staphylococcus aureus was most commonly isolated among all culture positive isolates. Conclusion: This study gives an outline of antibiotic susceptibility pattern of clinical isolates causing pyogenic wound infections which will help in empirical treatment of patients.

Keywords

• multidrug resistant
• pyogenic infections
• bacteriological profile