The Potential of <i>Alternaria</i> Toxins Production by <i>A</i>. <i>alternata</i> in Processing Tomatoes
Qiaomei Qin,
Yingying Fan,
Qinlan Jia,
Shuaishuai Duan,
Fengjuan Liu,
Binxin Jia,
Guangquan Wang,
Wanhui Guo,
Cheng Wang
Affiliations
Qiaomei Qin
College of Life Science and Technology, Xinjiang University, Urumqi 830049, China
Yingying Fan
Key Laboratory of Agro-products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agri-products (Urumqi), Institute of Quality Standards & Testing Technology for Agri-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
Qinlan Jia
College of Life Science and Technology, Xinjiang University, Urumqi 830049, China
Shuaishuai Duan
College of Life Science and Technology, Xinjiang University, Urumqi 830049, China
Fengjuan Liu
Key Laboratory of Agro-products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agri-products (Urumqi), Institute of Quality Standards & Testing Technology for Agri-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
Binxin Jia
Key Laboratory of Agro-products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agri-products (Urumqi), Institute of Quality Standards & Testing Technology for Agri-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
Guangquan Wang
College of Biology and Geography Sciences, Yili Normal University, Yining 835000, China
Wanhui Guo
Key Laboratory of Agro-products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agri-products (Urumqi), Institute of Quality Standards & Testing Technology for Agri-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
Cheng Wang
College of Life Science and Technology, Xinjiang University, Urumqi 830049, China
As a filamentous and spoilage fungus, Alternaria spp. can not only infect processing tomatoes, but also produce a variety of mycotoxins which harm the health of human beings. To explore the production of Alternaria toxins in processing tomatoes during growth and storage, four main Alternaria toxins and four conjugated toxins were detected by ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and ultra-performance liquid chromatography-ion mobility quadrupole time-of-flight mass spectrometry (UPLC-IMS QToF MS) in processing tomatoes on different days after being inoculated with A. alternata. The results show that the content of Alternaria toxins in an in vivo assay is higher than that under field conditions. Tenuazonic acid (TeA) is the predominant toxin detected in the field (205.86~41,389.19 μg/kg) and in vivo (7.64~526,986.37 μg/kg) experiments, and the second-most abundant toxin is alternariol (AOH). In addition, a small quantity of conjugated toxins, AOH-9-glucoside (AOH-9-Glc) and alternariol monomethyl ether-3-glucoside (AME-3-Glc), were screened in the in vivo experiment. This is the first time the potential of Alternaria toxins produced in tomatoes during the harvest period has been studied in order to provide data for the prevention and control of Alternaria toxins.
