A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus

Mike Flint; Payel Chatterjee; David L. Lin; Laura K. McMullan; Punya Shrivastava-Ranjan; Éric Bergeron; Michael K. Lo; Stephen R. Welch; Stuart T. Nichol; Andrew W. Tai; Christina F. Spiropoulou

doi:10.1038/s41467-018-08135-4

Nature Communications (Jan 2019)

A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus

Mike Flint,
Payel Chatterjee,
David L. Lin,
Laura K. McMullan,
Punya Shrivastava-Ranjan,
Éric Bergeron,
Michael K. Lo,
Stephen R. Welch,
Stuart T. Nichol,
Andrew W. Tai,
Christina F. Spiropoulou

Affiliations

Mike Flint: Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention
Payel Chatterjee: Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention
David L. Lin: Department of Microbiology & Immunology, University of Michigan Medical School
Laura K. McMullan: Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention
Punya Shrivastava-Ranjan: Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention
Éric Bergeron: Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention
Michael K. Lo: Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention
Stephen R. Welch: Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention
Stuart T. Nichol: Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention
Andrew W. Tai: Department of Microbiology & Immunology, University of Michigan Medical School
Christina F. Spiropoulou: Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention

DOI: https://doi.org/10.1038/s41467-018-08135-4
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 13

Abstract

Read online

Genetic screens are important tools to identify host factors associated with viral infections. Here, Flint et al. perform a genome-wide CRISPR screen using infectious Ebola virus (EBOV) and show that the host transferase GNPTAB is required for EBOV infection and a potential target for antiviral therapies

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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