Representation of microorganisms of subgingival plaque at different degrees of periodontium tissue inflamation and destruction

Abstract

Parodontopathy is an inflammatory reaction to gram negative anaerobic bacterial infectious agents that attacks the supporting dental apparatus including gingiva, periodontal ligament, cement and alveolar bone. The aim of the survey was to identify quantitative qualitative structure of microorganisms of subgingival plaque in patients suffering from chronic and aggressive parodontopathy using the PCR method, and then evaluate the correlation of different degrees of inflammation and destruction of periodontium tissue with the presence and concentration of these microorganisms. The survey involved 70 patients, 16 to 65 years old. The identification of microorganisms in subgingival plaque was set by the PCR method (Polymerase Chain Reaction). Towards diagnosing and defining the destruction degree of periodontal tissue, standard epidemiological criteria were used: plaque index (Silness-Löe), gingival index (Löe-Silness), SBI index (Mühleman-Son) and PDDZ. The presence of periodontal pathogens in subgingival plaque showed the statistical link with clinical parameters of the severeness of parodontopathy and gingival inflammation. The test result of Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans statistically was significantly more frequent in patients with medium and severe type of parodontopathy compared to the average depth of periodontal pockets. The detection of P.g. and A.a. statistically was significantly more frequent in persons with mild and intensive gingival inflammation.

Keywords

• parodontopathy
• subgingival plaque
• PCR