A new enzyme-linked immunosorbent assay for serological diagnosis of seal parapoxvirus infection in marine mammals

Badr Yassien; Rahman Md. Matiur; Ohno Yoshito; Ishijima Keita; Maeda Ken; Kohyama Kaoru; Kamatari Yuji O.; Shimizu Kaori; Okada Ayaka; Inoshima Yasuo

doi:10.2478/jvetres-2022-0005

Journal of Veterinary Research (Mar 2022)

A new enzyme-linked immunosorbent assay for serological diagnosis of seal parapoxvirus infection in marine mammals

Badr Yassien,
Rahman Md. Matiur,
Ohno Yoshito,
Ishijima Keita,
Maeda Ken,
Kohyama Kaoru,
Kamatari Yuji O.,
Shimizu Kaori,
Okada Ayaka,
Inoshima Yasuo

Affiliations

Badr Yassien: Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Damanhour, Egypt
Rahman Md. Matiur: Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Damanhour, Egypt
Ohno Yoshito: Port of Nagoya Public Aquarium, Minato-ku, Nagoya, Aichi 455-0033, Japan
Ishijima Keita: Department of Veterinary Science, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan
Maeda Ken: Department of Veterinary Science, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan
Kohyama Kaoru: Izu Mito Sea Paradise, Numazu, Shizuoka 410-0224, Japan
Kamatari Yuji O.: Division of Instrumental Analysis, Life Science Research Center, Yamaguchi, Japan
Shimizu Kaori: Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Damanhour, Egypt
Okada Ayaka: Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Damanhour, Egypt
Inoshima Yasuo: Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Damanhour, Egypt

DOI: https://doi.org/10.2478/jvetres-2022-0005
Journal volume & issue: Vol. 66, no. 1
pp. 43 – 52

Abstract

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Seal parapoxvirus (SPPV) infection has been reported among pinnipeds in aquaria in Japan; however, its seroprevalence is unknown. Therefore, an enzyme-linked immunosorbent assay (ELISA) was developed for serological diagnosis of SPPV infection.

Published in Journal of Veterinary Research

ISSN: 2450-8608 (Online)
Publisher: Sciendo
Country of publisher: Poland
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: https://sciendo.com/journal/JVETRES

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