STAR Protocols (Mar 2024)

Protocol for sorting and culturing of mouse early erythroid progenitor BFU-E cells

  • Yao Li,
  • Ziyu Liang,
  • Hailin Wang

Journal volume & issue
Vol. 5, no. 1
p. 102718

Abstract

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Summary: Techniques allowing the long-term culture of the burst-forming unit of erythroid (BFU-E) progenitor cells are essential for understanding erythropoiesis. Here, we present a protocol for sorting mouse BFU-E cells and culturing them in a medium that promotes BFU-E cell expansion. We describe steps for isolating BFU-E cells from mouse fetal livers by combining magnetic microbeads with flow cytometry and culturing BFU-E cells with a specific expansion media. This approach can enhance the production of BFU-E cells.For complete details on the use and execution of this protocol, please refer to Li et al..1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

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