The effect of high temperature on chemical structure of red blood corpuscles membranes for employees in ovens of Ramadi glass factory – Iraq.

Abstract

This study include investigation the effects of high temperature on the chemical structure of RBCs membranes in thirty workers of the state coperation of glass industries in Ramadi - Iraq , thirty technicians and administrators for interior control and thirty volunteers as external control . RBCs membranes isolation and characterization of lipids and protiens and checking them by using TLC and gel electrophoresis respectively . From the results of this study we observed proteins of RBCs membrane decreased significantly (P<0.01) , cholesterol and phospholipids increased significantly (P<0.01) , (lipidsproteins) ratio increased significantly (P<0.01) . RBCs membranes proteins electrophoresis on (polyacrylamide gel) showed the disappearance of number of proteins bands and decreased the density of the other bands , while the separation on the (TLC) showed that there were clear differences and there was change in the classes of RBCs membrane lipids.