Communications Biology (Feb 2021)

Rapid whole cell imaging reveals a calcium-APPL1-dynein nexus that regulates cohort trafficking of stimulated EGF receptors

  • H. M. York,
  • A. Patil,
  • U. K. Moorthi,
  • A. Kaur,
  • A. Bhowmik,
  • G. J. Hyde,
  • H. Gandhi,
  • A. Fulcher,
  • K. Gaus,
  • S. Arumugam

DOI
https://doi.org/10.1038/s42003-021-01740-y
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 13

Abstract

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York et al employ lattice light-sheet microscopy to study the initial events following activation of the epidermal growth factor receptor (EGFR) in whole live cells. They find that APPL1 relocates from pre-existing endosomes, triggered by calcium, to EGFR puncta which results in a cohort, dynein mediated movement of EGFR towards the peri-nuclear region of the cell, expanding our understanding of EGFR trafficking dynamics.