Chemical Profiling, Antioxidant, and Antimicrobial Activity against Drug-Resistant Microbes of Essential Oil from <i>Withania frutescens</i> L.

Abdelfattah EL Moussaoui; Mohammed Bourhia; Fatima Zahra Jawhari; Ahmad Mohammad Salamatullah; Riaz Ullah; Ahmed Bari; Hafiz Majid Mahmood; Muhammad Sohaib; Bohza Serhii; Alexander Rozhenko; Mourad A. M. Aboul-Soud; Essam Ezzeldin; Gamal A. E. Mostafa; Dalila Bousta; Amina Bari

doi:10.3390/app11115168

Applied Sciences (Jun 2021)

Chemical Profiling, Antioxidant, and Antimicrobial Activity against Drug-Resistant Microbes of Essential Oil from <i>Withania frutescens</i> L.

Abdelfattah EL Moussaoui,
Mohammed Bourhia,
Fatima Zahra Jawhari,
Ahmad Mohammad Salamatullah,
Riaz Ullah,
Ahmed Bari,
Hafiz Majid Mahmood,
Muhammad Sohaib,
Bohza Serhii,
Alexander Rozhenko,
Mourad A. M. Aboul-Soud,
Essam Ezzeldin,
Gamal A. E. Mostafa,
Dalila Bousta,
Amina Bari

Affiliations

Abdelfattah EL Moussaoui: Laboratory of Biotechnology, Environment, Agrifood and Health, Faculty of Science, University of Sidi Mohamed Ben Abdellah, Fez 30050, Morocco
Mohammed Bourhia: Laboratory of Chemistry-Biochemistry, Environment, Nutrition and Health, Faculty of Medicine and Pharmacy, Hassan II University, Casablanca 21100, Morocco
Fatima Zahra Jawhari: Laboratory of Biotechnology, Environment, Agrifood and Health, Faculty of Science, University of Sidi Mohamed Ben Abdellah, Fez 30050, Morocco
Ahmad Mohammad Salamatullah: Department of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia
Riaz Ullah: Department of Pharmacognosy (MAPPRC), College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Ahmed Bari: Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Hafiz Majid Mahmood: Department of Pharmacology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
Muhammad Sohaib: Department of Soil Sciences, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia
Bohza Serhii: Institute of Organic Chemistry of the National Academy of Sciences of Ukraine, Murmans’ka str. 5, 02660 Kyiv, Ukraine
Alexander Rozhenko: Institute of Organic Chemistry of the National Academy of Sciences of Ukraine, Murmans’ka str. 5, 02660 Kyiv, Ukraine
Mourad A. M. Aboul-Soud: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia
Essam Ezzeldin: Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Gamal A. E. Mostafa: Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Dalila Bousta: Laboratory of Biotechnology, Environment, Agrifood and Health, Faculty of Science, University of Sidi Mohamed Ben Abdellah, Fez 30050, Morocco
Amina Bari: Laboratory of Biotechnology, Environment, Agrifood and Health, Faculty of Science, University of Sidi Mohamed Ben Abdellah, Fez 30050, Morocco

DOI: https://doi.org/10.3390/app11115168
Journal volume & issue: Vol. 11, no. 11
p. 5168

Abstract

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This work was conducted to study the chemical composition, antioxidant, antibacterial, and antifungal activities of essential oil and hydrolat from Withania frutescens. The essential oil was extracted by hydrodistillation. The chemical characterization was performed using gas chromatography-mass spectrometry (GC/MS). The antioxidant activity was studied using four different assays (DPPH, TAC, FRAP, and β-carotene bleaching). The antibacterial activity test was carried out on multidrug-resistant bacteria including Gram-negative and Gram-positive strains. Antifungal activity was tested on Candida albicans and Saccharomyces cerevisiae. The yield of essential oil (EO) obtained by hydrodistillation of W. frutescens was 0.31% majorly composed of camphor, α-thujone, carvacrol, and thymol. Regarding the antioxidant activities, the concentration of the sample required to inhibit 50% of radicals (IC50) of EO and hydrolat were 14.031 ± 0.012 and 232.081 ± 3.047 µg/mL (DPPH), 4.618 ± 0.045 and 8.997 ± 0.147 µg/mL (FRAP), 0.091 ± 0.007 and 0.131 ± 0.004 mg AAE/mg (TAC), 74.141 ± 1.040% and 40.850 ± 0.083% (β-carotene), respectively. Concerning the antibacterial activity of essential oil and hydrolat, the minimum inhibitory concentration (MIC) values found were 0.006 ± 0.001 and 6.125 ± 0.541 µg/mL (Escherichia coli 57), 0.003 ± 0.001 and 6.125 ± 0.068 µg/mL (Klebsiella pneumoniae), 0.001 ± 0.0 and 6.125 ± 0.046 µg/mL (Pseudomonas aeruginosa) and 0.012 ± 0.003 and 6.125 ± 0.571 µg/mL (Staphylococcus aureus), respectively. MIC values of essential oil and hydrolat vs. both C. albicans and S. cerevisiae were lower than 1/20,480 µg/mL. Based on the findings obtained, essential oils of Withania frutescens can be used as promising natural agents to fight free radical damage and nosocomial antibiotic-resistant microbes.

Published in Applied Sciences

ISSN: 2076-3417 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Engineering (General). Civil engineering (General); Science: Biology (General); Science: Physics; Science: Chemistry
Website: http://www.mdpi.com/journal/applsci

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