Electronic Journal of Plant Breeding (Jul 2010)
Genetic Diversity Assessment Across Different Genotypes Of Mungbean And Urdbean Using Molecular Markers
Abstract
Pulses compliment the daily diet of Indians along with cereals. They are rich in proteins with satisfactory proportion ofcarbohydrates. Mungbean, Vigna radiata and Urd bean, Vigna mungo are the important grain legume crops in agriculture,particularly in India. MYMV (Mungbean Yellow vein Mosaic Virus) is a virus transmitted by whitefly, Bemesia tabaci, themost serious disease of Mungbean and Urdbean. In this study, six each of MYMV resistant and susceptible genotypes inMungbean and Urbean respectively were selected for the diversity analysis using molecular markers. Twenty four RGAprimers from cowpea were used to screen the twenty four genotypes. Dendrogram generated clearly indicated two bigclusters at 15% similarity. All mungbean genotypes made one cluster (cluster A) except PS16, which was included in othercluster made by Urdbean genotypes (cluster B). Cluster A contained eleven genotypes while cluster B contained thirteengenotypes. Cluster A and B were further classified into two sub clusters namely A1 and A2, B1 and B2 respectively. A1consisted of seven genotypes of which five were resistant (PANT MUNG 1, PANT MUNG 5, HUM 12, PUSA 9531, HUM1) and two were susceptible (TARM 2, KOPERGAON 3), while A2 comprised of remaining four genotypes in which threewere susceptible (TAP 7, SML 134 and SML 668), and one (AKM 8803) was resistant. Further, it was found that fourmungbean resistant genotypes of A1 namely Pant Mung1, Pant Mung5, HUM 12, and PUSA 9531 made one cluster at 55%similarity. Cluster B, again was subdivided into B1 and B2. B1 consisted a single genotype which was a cross between IPU99-25* SPS5 while, B2 consisted of the rest of the twelve genotypes. It was interesting to see that two resistant (IPU 02-33and IPU 6-02) and two susceptible (LBG 20 and T9) genotypes of Urd bean made separate cluster with a similarity of 99 percent and which indicated that though genotypes are differing at resistant locus, they are highly similar at all other loci.
