Qualitative and Quantitative Analysis of <i>C</i>-glycosyl-flavones of <i>Iris lactea</i> Leaves by Liquid Chromatography/Tandem Mass Spectrometry
Dan Chen,
Yu Meng,
Yan Zhu,
Gang Wu,
Jun Yuan,
Minjian Qin,
Guoyong Xie
Affiliations
Dan Chen
Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, #24 Tongjiaxiang, Gulou District, Nanjing 210009, China
Yu Meng
Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, #24 Tongjiaxiang, Gulou District, Nanjing 210009, China
Yan Zhu
Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, #24 Tongjiaxiang, Gulou District, Nanjing 210009, China
Gang Wu
Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, #24 Tongjiaxiang, Gulou District, Nanjing 210009, China
Jun Yuan
Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huai’an 223003, Jiangsu, China
Minjian Qin
Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, #24 Tongjiaxiang, Gulou District, Nanjing 210009, China
Guoyong Xie
Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, #24 Tongjiaxiang, Gulou District, Nanjing 210009, China
Iris lactea Pall. var. chinensis (Fisch.) Koidz. is a traditional medicinal plant resource. To make full use of the I. lactea plant resources, constituents of I. lactea leaves were determined by high performance liquid chromatography (HPLC)-quadrupole time-of-flight tandem mass spectrometry and 22 C-glycosylflavones were identified or tentatively identified. Optimal extraction of I. lactea leaves was established via single factor investigations combined with response surface methodology. Then, HPLC coupled with a diode array detector was used to quantitatively analyze the six main components of 14 batches of I. lactea leaves grown in different areas. The results showed the C-glycosylflavones were the main components of I. lactea leaves, and the total contents of detected components were relatively stable for the majority of samples. These results provide a foundation for the development and utilization of I. lactea leaves.
