Light Guided In-vivo Activation of Innate Immune Cells with Photocaged TLR 2/6 Agonist

Keun Ah Ryu; Bethany McGonnigal; Troy Moore; Tawnya Kargupta; Rock J. Mancini; Aaron P. Esser-Kahn

doi:10.1038/s41598-017-08520-x

Scientific Reports (Aug 2017)

Light Guided In-vivo Activation of Innate Immune Cells with Photocaged TLR 2/6 Agonist

Keun Ah Ryu,
Bethany McGonnigal,
Troy Moore,
Tawnya Kargupta,
Rock J. Mancini,
Aaron P. Esser-Kahn

Affiliations

Keun Ah Ryu: Department of Chemistry, University of California, Irvine
Bethany McGonnigal: Department of Chemistry, University of California, Irvine
Troy Moore: Department of Chemistry, University of California, Irvine
Tawnya Kargupta: Department of Chemistry, University of California, Irvine
Rock J. Mancini: Department of Chemistry, University of California, Irvine
Aaron P. Esser-Kahn: Department of Chemistry, University of California, Irvine

DOI: https://doi.org/10.1038/s41598-017-08520-x
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 7

Abstract

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Abstract The complexity of the immune system creates challenges in exploring its importance and robustness. To date, there have been few techniques developed to manipulate individual components of the immune system in an in vivo environment. Here we show a light-based dendritic cell (DC) activation allowing spatial and temporal control of immune activation in vivo. Additionally, we show time dependent changes in RNA profiles of the draining lymph node, suggesting a change in cell profile following DC migration and indicating that the cells migrating have been activated towards antigen presentation.

Published in Scientific Reports

ISSN: 2045-2322 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://www.nature.com/srep/

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