Brazilian Archives of Biology and Technology (Jun 2020)

Antibacterial Activity of Sophorolipids from Candida bombicola Against Human Pathogens

  • Isadora Cernach Carneiro da Fontoura,
  • Gustavo Issamu Asai Saikawa,
  • Vitória Akemi Itakura Silveira,
  • Nicole Caldas Pan,
  • Ismael Rodrigues Amador,
  • Cristiani Baldo,
  • Sérgio Paulo Dejato da Rocha,
  • Maria Antonia Pedrine Colabone Celligoi

DOI
https://doi.org/10.1590/1678-4324-2020180568
Journal volume & issue
Vol. 63

Abstract

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Abstract Sophorolipids are glycolipids that have natural antimicrobial properties and present great potential in the pharmaceutical field. The present study aimed to produce sophorolipids from Candida bombicola using a chicken fat-based medium and evaluate the antimicrobial activity against Gram-negative (Proteus mirabilis, Escherichia coli, Salmonella enterica subsp. enterica) and Gram-positive bacteria (Enterococcus faecium, Staphylococcus aureus and Streptococcus mutans). The production of sophorolipids reached 27.86 g L-1. Based on the structural characterization, 73.55% of the sophorolipids present a mixture of acidic monoacetylated C18:2 and lactonic diacetylated C16:0, and 26.45% were present in the diacetylated C18:1 lactonic form. Bacteria submitted to sophorolipid exposure showed a reduction in viability at doses of 500 μg mL-1 and 2,000 μg mL-1 against Gram-positive and Gram-negative bacteria, respectively. These results suggest that sophorolipids produced in chicken fat medium may be used as antimicrobial agents to prevent or eliminate contamination by different pathogens.

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