Nature Communications (Aug 2021)
Translational control by DHX36 binding to 5′UTR G-quadruplex is essential for muscle stem-cell regenerative functions
- Xiaona Chen,
- Jie Yuan,
- Guang Xue,
- Silvia Campanario,
- Di Wang,
- Wen Wang,
- Xi Mou,
- Shiau Wei Liew,
- Mubarak Ishaq Umar,
- Joan Isern,
- Yu Zhao,
- Liangqiang He,
- Yuying Li,
- Christopher J. Mann,
- Xiaohua Yu,
- Lei Wang,
- Eusebio Perdiguero,
- Wei Chen,
- Yuanchao Xue,
- Yoshikuni Nagamine,
- Chun Kit Kwok,
- Hao Sun,
- Pura Muñoz-Cánoves,
- Huating Wang
Affiliations
- Xiaona Chen
- Department of Orthopaedics and Traumatology, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong
- Jie Yuan
- Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong
- Guang Xue
- Department of Orthopaedics and Traumatology, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong
- Silvia Campanario
- Department of Experimental & Health Sciences, Universitat Pompeu Fabra (UPF), CIBERNED, ICREA
- Di Wang
- Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences
- Wen Wang
- Department of Biology, Southern University of Science and Technology
- Xi Mou
- Department of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong Kong, Kowloon Tong
- Shiau Wei Liew
- Department of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong Kong, Kowloon Tong
- Mubarak Ishaq Umar
- Department of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong Kong, Kowloon Tong
- Joan Isern
- Department of Experimental & Health Sciences, Universitat Pompeu Fabra (UPF), CIBERNED, ICREA
- Yu Zhao
- Department of Orthopaedics and Traumatology, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong
- Liangqiang He
- Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong
- Yuying Li
- Department of Orthopaedics and Traumatology, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong
- Christopher J. Mann
- Department of Experimental & Health Sciences, Universitat Pompeu Fabra (UPF), CIBERNED, ICREA
- Xiaohua Yu
- Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences
- Lei Wang
- Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences
- Eusebio Perdiguero
- Department of Experimental & Health Sciences, Universitat Pompeu Fabra (UPF), CIBERNED, ICREA
- Wei Chen
- Department of Biology, Southern University of Science and Technology
- Yuanchao Xue
- Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences
- Yoshikuni Nagamine
- Friedrich Miescher Institute for Biomedical Research, Novartis Research Foundation
- Chun Kit Kwok
- Department of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong Kong, Kowloon Tong
- Hao Sun
- Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong
- Pura Muñoz-Cánoves
- Department of Experimental & Health Sciences, Universitat Pompeu Fabra (UPF), CIBERNED, ICREA
- Huating Wang
- Department of Orthopaedics and Traumatology, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong
- DOI
- https://doi.org/10.1038/s41467-021-25170-w
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 22
Abstract
Skeletal muscle stem cells (or satellite cells, SCs) are normally quiescent but activate and expand in response to injury. Here the authors show that induction of DHX36 helicase during SC activation promotes mRNA translation by binding to 5′UTR mRNA G-quadruplexes (rG4) in targets including Gnai2 and unwinding them.
