In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix

Jiyoung Lee; Akito Sutani; Rin Kaneko; Jun Takeuchi; Tetsuo Sasano; Takashi Kohda; Kensuke Ihara; Kentaro Takahashi; Masahiro Yamazoe; Tomohiro Morio; Tetsushi Furukawa; Fumitoshi Ishino

doi:10.1038/s41467-020-18031-5

Nature Communications (Sep 2020)

In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix

Jiyoung Lee,
Akito Sutani,
Rin Kaneko,
Jun Takeuchi,
Tetsuo Sasano,
Takashi Kohda,
Kensuke Ihara,
Kentaro Takahashi,
Masahiro Yamazoe,
Tomohiro Morio,
Tetsushi Furukawa,
Fumitoshi Ishino

Affiliations

Jiyoung Lee: Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University (TMDU)
Akito Sutani: Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University (TMDU)
Rin Kaneko: Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University (TMDU)
Jun Takeuchi: Department of Bio-Informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University (TMDU)
Tetsuo Sasano: Department of Cardiovascular Medicine, Tokyo Medical and Dental University (TMDU)
Takashi Kohda: Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University (TMDU)
Kensuke Ihara: Department of Bio-Informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University (TMDU)
Kentaro Takahashi: Department of Bio-Informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University (TMDU)
Masahiro Yamazoe: Department of Bio-Informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University (TMDU)
Tomohiro Morio: Department of Pediatrics and Developmental Biology, Tokyo Medical and Dental University (TMDU)
Tetsushi Furukawa: Department of Bio-Informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University (TMDU)
Fumitoshi Ishino: Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University (TMDU)

DOI: https://doi.org/10.1038/s41467-020-18031-5
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 18

Abstract

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Our understanding of the development of the heart has been limited by a lack of in vitro cellular models. Here, the authors treat mouse embryonic stem cell-derived embryoid bodies with laminin-entactin (to mimic the developing microenvironment) and FGF4 to form heart organoids, with atrial and ventricular-like parts.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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