Nature Communications (May 2020)

mRNA display with library of even-distribution reveals cellular interactors of influenza virus NS1

  • Yushen Du,
  • Judd F. Hultquist,
  • Quan Zhou,
  • Anders Olson,
  • Yenwen Tseng,
  • Tian-hao Zhang,
  • Mengying Hong,
  • Kejun Tang,
  • Liubo Chen,
  • Xiangzhi Meng,
  • Michael J. McGregor,
  • Lei Dai,
  • Danyang Gong,
  • Laura Martin-Sancho,
  • Sumit Chanda,
  • Xinming Li,
  • Steve Bensenger,
  • Nevan J. Krogan,
  • Ren Sun

DOI
https://doi.org/10.1038/s41467-020-16140-9
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 13

Abstract

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Identification of low abundance proteins interacting with viral proteins can be challenging. Here, Du et al. develop an mRNA display approach with a library of even distribution, identify host proteins interacting with NS1 protein of influenza A virus, and show that one interactor provides a means to regulate cellular fatty acids synthesis.