Development of a MaizeGerm50K array and application to maize genetic studies and breeding
Honghui Guan,
Yaxuan Lu,
Xinchun Li,
Bei Liu,
Yongxiang Li,
Dengfeng Zhang,
Xuyang Liu,
Guanhua He,
Yu Li,
Hongwu Wang,
Tianyu Wang,
Chunhui Li
Affiliations
Honghui Guan
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yaxuan Lu
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Xinchun Li
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Bei Liu
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yongxiang Li
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Dengfeng Zhang
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Xuyang Liu
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Guanhua He
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yu Li
Corresponding authors.; State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Hongwu Wang
Corresponding authors.; State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Tianyu Wang
Corresponding authors.; State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Chunhui Li
Corresponding authors.; State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Genotyping arrays based on single nucleotide polymorphisms (SNPs) provide a low-cost, high-throughput platform. The development of a SNP array that fully reflects the genetic diversity of maize (Zea mays L.) germplasm and is applicable to molecular breeding programs is desirable. In this study, we developed a MaizeGerm50K array comprising 50,852 SNPs selected from the resequencing data of 1604 maize inbred lines and other markers. A genome-wide association study using a landrace panel genotyped with the array permitted mapping of several known genes. We also verified a candidate gene, RNA-binding motif protein 24-like 1 (ZmRBM24L1), delaying flowering through overexpression lines. Genomic selection for yield and agronomic traits showed high prediction accuracy. The MaizeGerm50K array is thus a valuable genomic tool for maize genetic studies and breeding.
