Trends in the indicators of lipid peroxidation and antioxidant protection of rat blood serum and heart under acute experimental lung injury

Abstract

Dynamically, acute experimental lung injury (ALI), caused by intratracheal injection of chloride acid, revealed one-direction disorder of free radical oxidation processes in the blood serum and heart homogenate, the intensity of the processes increasing within 12 and 24 experimental hour. However, 48 hours after ALI simulation the growing concentration of TBA-active products was found in the blood serum only, while in the heart homogenate the index was found to be decreasing. In 72 hours, the blood serum revealed insignificant decrease in TBA-active products’ concentration. However, the index was about 3 times the control value. In the heart homogenate the index was found to decrease 72 hours after experiment start and was reaching the level of the control group. Owing to ALI simulation, the catalase activity in the blood serum exceeds the control level throughout the experiment, peak values found in 24 hours, whereas in the heart homogenate the index was found to be gradually decreasing till the 48th hour, remaining significantly lower as compared to the control level. Nevertheless, in 72 hours the catalase activity in the heart homogenate grows and exceeds the control level notably. Analysis of the antioxidant-pro-oxidant balance that was evaluated in the blood serum and heart homogenate by the API value revealed significant growth of the index in the blood serum within 12 and 24 hours as compared to the controls. However, by 72 hour the index was found to be decreasing reliably below the control level. Meanwhile, in the heart homogenate the API value is below the control level throughout 48 hours since ALI simulation, growing rapidly further and exceeding the control level notably in 72 hours.

Keywords

• acute lung injury, lipid peroxidation, catalase, heart