Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum (May 2017)
Prevalence and Antibiotic Resistance of Staphylococcus aureus Isolated from Nose of Personnel of Operating Rooms of Nekouei Hospitalin Qom City,(Iran) 2014
Abstract
Background and Objectives: Staphylococcus aureus is one of the five causes of nosocomial infections. Presence of this bacterium in operating rooms is one of the most important causes of nosocomial infections. This study was performed to investigate the contamination rate of S. aureus in the nose of personnel of operating rooms and its related antibiotic resistance. Methods: In this study, samples were taken from the nasal entrance of the personnel using a sterile swab on Mueller-Hinton agar medium. Then, the obtained samples were transferred to mannitol salt agar, and catalase- and coagulase-positive Staphylococci, were separated. Finally, agar disk diffusion method was used to determine antibiotic susceptibility. Data analysis was performed using Chi square and t-test. Results: In this research, 47 males (60.25) and 31 females (39.75) were studied. Twenty-two subjects (28.20%) {9 (40.90%) females and 13 (59.09%) males}, were nasal carriers of Staphylococcus aureus. Five experimental bacteria carrier groups included 3 (13.63%) surgeons, 7 (83.31%) scrubs, 4 (18.18%) circulars, 4 (18.18%) anesthesias, 4 (18/18%) services working in operating room. Most antibiotic resistance in the nose was against penicillin and piperacillin with resistance of 20 samples (90%) and the lowest resistance was against vancomycin with resistance of 4 samples (18.18%) and cefotaxime with 5 samples (22.72%). Of five occupational categories, scrub staff were the highest and surgeons were the lowest nasal carriers. 31.83% and 13.63%, respectively). There was a significant relationship between occupational category and nasal staphylococcus. Conclusion: According to the results of this study, limited number of operating room staff were resistant to cefotaxime and vancomycin antibiotics. Therefore, more attention can be paid to adoption of therapeutic and some preventive methods by these two antibiotics and also recommended of excessive prescription of antibiotics is prevention.
