Non-Invasive measurement of the cerebral metabolic rate of oxygen using MRI in rodents [version 4; peer review: 2 approved]

Eilidh MacNicol; Diana Cash; Eugene Kim; Camilla Simmons; Fernando Zelaya; David J Lythgoe; Federico Turkheimer; Tobias C Wood

Wellcome Open Research (Aug 2022)

Non-Invasive measurement of the cerebral metabolic rate of oxygen using MRI in rodents [version 4; peer review: 2 approved]

Eilidh MacNicol,
Diana Cash,
Eugene Kim,
Camilla Simmons,
Fernando Zelaya,
David J Lythgoe,
Federico Turkheimer,
Tobias C Wood

Affiliations

Eilidh MacNicol: ORCiD; Department of Neuroimaging, Institute of Psychiatry, Psychology & Neuroscience, King's College London, SE5 8AF, UK
Diana Cash: Department of Neuroimaging, Institute of Psychiatry, Psychology & Neuroscience, King's College London, SE5 8AF, UK
Eugene Kim: ORCiD; Department of Neuroimaging, Institute of Psychiatry, Psychology & Neuroscience, King's College London, SE5 8AF, UK
Camilla Simmons: ORCiD; Department of Neuroimaging, Institute of Psychiatry, Psychology & Neuroscience, King's College London, SE5 8AF, UK
Fernando Zelaya: Department of Neuroimaging, Institute of Psychiatry, Psychology & Neuroscience, King's College London, SE5 8AF, UK
David J Lythgoe: Department of Neuroimaging, Institute of Psychiatry, Psychology & Neuroscience, King's College London, SE5 8AF, UK
Federico Turkheimer: ORCiD; Department of Neuroimaging, Institute of Psychiatry, Psychology & Neuroscience, King's College London, SE5 8AF, UK
Tobias C Wood: ORCiD; Department of Neuroimaging, Institute of Psychiatry, Psychology & Neuroscience, King's College London, SE5 8AF, UK

Journal volume & issue: Vol. 6

Abstract

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Malfunctions of oxygen metabolism are suspected to play a key role in a number of neurological and psychiatric disorders, but this hypothesis cannot be properly investigated without an in-vivo non-invasive measurement of brain oxygen consumption. We present a new way to measure the Cerebral Metabolic Rate of Oxygen (CMRO2) by combining two existing magnetic resonance imaging techniques, namely arterial spin-labelling and oxygen extraction fraction mapping. This method was validated by imaging rats under different anaesthetic regimes and was strongly correlated to glucose consumption measured by autoradiography.

Published in Wellcome Open Research

ISSN: 2398-502X (Online)
Publisher: Wellcome
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://wellcomeopenresearch.org/

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