A Versatile catalytic and photothermal lateral flow immunoassay Based on ultrathin Fe‐MoS2 nanosheets for sensitive and accurate detection of Influenza A
Meimei Xu,
Chenglong Lin,
Shuai Zhao,
Weida Zhang,
Dan Li,
Fanghao Fang,
Zheng Teng,
Yusi Peng,
Ming Liu,
Zhengren Huang,
Jianlin Shi,
Yong Yang
Affiliations
Meimei Xu
State Key Laboratory of High‐Performance Ceramics and Superfine Microstructures Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai P. R. China
Chenglong Lin
State Key Laboratory of High‐Performance Ceramics and Superfine Microstructures Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai P. R. China
Shuai Zhao
State Key Laboratory of High‐Performance Ceramics and Superfine Microstructures Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai P. R. China
Weida Zhang
State Key Laboratory of High‐Performance Ceramics and Superfine Microstructures Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai P. R. China
Dan Li
State Key Laboratory of High‐Performance Ceramics and Superfine Microstructures Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai P. R. China
Fanghao Fang
Virus Testing Laboratory Shanghai Municipal Center for Disease Control and Prevention Shanghai P. R. China
Zheng Teng
Virus Testing Laboratory Shanghai Municipal Center for Disease Control and Prevention Shanghai P. R. China
Yusi Peng
State Key Laboratory of High‐Performance Ceramics and Superfine Microstructures Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai P. R. China
Ming Liu
Department of Orthopedics Shanghai Fourth People's Hospital School of Medicine Tongji University Shanghai P. R. China
Zhengren Huang
State Key Laboratory of High‐Performance Ceramics and Superfine Microstructures Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai P. R. China
Jianlin Shi
State Key Laboratory of High‐Performance Ceramics and Superfine Microstructures Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai P. R. China
Yong Yang
State Key Laboratory of High‐Performance Ceramics and Superfine Microstructures Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai P. R. China
Abstract Influenza A virus (H1N1) poses a significant threat to global human health that imperative demands the development of sensitive and accurate point‐of‐care testing (POCT) methods. Here, for the first time, Fe‐MoS2 nanosheets were employed as a multifunctional nanotag in the development of catalytic colorimetric‐photothermal dual‐mode lateral flow immunoassay (dLFIA) strips for the sensitive detection of H1N1 inactivated virus. The Fe‐MoS2 nanosheets featuring large size, high specific surface area, and ultrathin structure could flow smoothly on the strips and thus quickly produce an ideal colorimetric signal for qualitative analysis. Both the limit of detection (LOD) of catalytic colorimetric and photothermal signals reached 1000 copies/mL and the corresponding calculated LOD was 550 and 691 copies/mL, respectively, which were about 50–90‐fold more sensitive than traditional gold nanoparticles based‐LFIA (5 × 104 copies/mL). The developed assay could correctly identify eight positive clinical samples with Ct values less than 35 and 10 negative actual samples, proving significant promise for rapid, sensitive, and accurate detection of H1N1, especially in resource‐limited areas.
