ISOLATION OF SECONDARY METABOLITE FROM n -HEXANE EXTRACT OF THE STEM OF BROTOWALI (Tinospora crispa)

Abstract

Isolation of secondary metabolite from n-hexane extract of the stem of Tinospora crispa had been studied. The extraction was performed by maceration. The extract of n-hexane was separated and purified by column chromatography. Antioxidant activity of the isolated compound was conducted by DPPH (1.1-diphenylpycryl hidrazyl) method with concentration variation of 1000, 500, 250, 125, 62.5, 31.25, 15.625 and 7.8125 μg/mL. The isolated compound was a yellow oils. Based on spectroscopy data 1D-NMR and comparing with literature data which had been reported from endophityc fungi of brotowali stem, it was concluded that the isolated compound was bis-(2-ethylhexyl) phthalate with molecular formula C24O4H38. The antioxidant activity of the isolated compound showed IC50 232.9 μg/mL and was inactive as antioxidant

Keywords

• Antioxidant
• bis-(2-ethylhexyl) phthalate
• Tinospora crispa